ARG55964
anti-Cytokeratin 14 antibody [LL002]
anti-Cytokeratin 14 antibody [LL002] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Elephant,Pig
概述
| 产品描述 | Mouse Monoclonal antibody [LL002] recognizes Cytokeratin 14 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Elp, Pig |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LL002 |
| 同位型 | IgG3 |
| 靶点名称 | Cytokeratin 14 |
| 抗原物种 | Human |
| 抗原 | A 15-mer synthetic peptide from the C-terminus of Human Cytokeratin 14. |
| 偶联标记 | Un-conjugated |
| 別名 | K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14 |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||
| 实际分子量 | ~ 53 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT14 |
| 全名 | keratin 14, type I |
| 背景介绍 | This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008] |
| 生物功能 | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
检测图片 (5) Click the Picture to Zoom In
-
ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Human prostate (10X) stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
-
ARG55964 anti-Cytokeratin 14 antibody [LL002] WB image
Western blot: A431 cell lysate (epidermoid carcinoma) stained with ARG55964 anti-Cytokeratin 14 antibody [LL002] at 1 µg/ml dilution.
-
ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Human prostate (20X) stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
-
ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
-
ARG55964 anti-Cytokeratin 14 antibody [LL002] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human prostate stained with ARG55964 anti-Cytokeratin 14 antibody [LL002].
