ARG53927
anti-Cytokeratin 18 antibody [DC 10] (FITC)
anti-Cytokeratin 18 antibody [DC 10] (FITC) for Flow cytometry and Human
Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [DC 10] recognizes Cytokeratin 18 |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat, Bov, Dog, Hm, Pig, Sheep |
| 应用 | FACS |
| 特异性 | The clone DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | DC 10 |
| 同位型 | IgG1 |
| 靶点名称 | Cytokeratin 18 |
| 抗原物种 | Human |
| 抗原 | Human breast carcinoma cell line PMC-42. |
| 偶联标记 | FITC |
| 別名 | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT18 |
| 全名 | keratin 18, type I |
| 背景介绍 | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
检测图片 (1) Click the Picture to Zoom In
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ARG53927 anti-Cytokeratin 18 antibody [DC 10] (FITC) FACS image
Flow Cytometry: HeLa cells stained with ARG53927 anti-Cytokeratin 18 antibody [DC 10] (FITC).
克隆号文献
Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.
WB / Mouse
Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
IHC / Human
Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.
WB / Human
Endometrial profile of tamoxifen and low-dose estradiol combination therapy.
IHC / Primates
Directed differentiation of human embryonic stem cells to epidermal progenitors.
FACS / Human
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
WB / Human
Mouse mammary tumor virus p75 and p110 CUX1 transgenic mice develop mammary tumors of various histologic types.
WB / Mouse
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
ICC/IF / Human
Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.
ELISA / Human
Mammary gland development in early pubertal female macaques.
IHC / Rhesus Monkey
Epithelial phenotypes in the developing human prostate.
IHC-P / Human
