ARG66248
anti-Cytokeratin 20 antibody [SQab1737]
anti-Cytokeratin 20 antibody [SQab1737] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
CK7/CK20 Carcinoma Study antibody
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1737] recognizes Cytokeratin 20 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1737 |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 20 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human Cytokeratin 20. |
| 偶联标记 | Un-conjugated |
| 別名 | KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT20 |
| 全名 | keratin 20, type I |
| 背景介绍 | Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30318 CK7 / CK20 Carcinoma Antibody Duo Related products: Cytokeratin 20 antibodies; Cytokeratin 20 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer |
| 研究领域 | CK7/CK20 Carcinoma Study antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG66248 anti-Cytokeratin 20 antibody [SQab1737] ICC/IF image
Immunofluorescence: HT-29 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at RT. Samples were stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (green) at 1:200, 4°C.
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ARG66248 anti-Cytokeratin 20 antibody [SQab1737] FACS image
Flow Cytometry: HT-29 cells stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the primary antibody at 1:1000 dilution in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat anti-rabbit Alexa Fluor® 488 (IgG H+L) at 1:2000 dilution for 20 min at 4°C. Unlabelled sample (Blue) was used as a control.
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ARG66248 anti-Cytokeratin 20 antibody [SQab1737] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded colonic adenocarcinoma stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:800. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
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ARG66248 anti-Cytokeratin 20 antibody [SQab1737] WB image
Western blot: 10 μg of HCT-116 and HT-29 cell lysates stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:5000 dilution.
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ARG66248 anti-Cytokeratin 20 antibody [SQab1737] IP image
Immunoprecipitation: Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:50 dilution.
1. IP by using ARG66248 in HT-29 whole cell lysate
2. PBS instead of ARG66248 in HT-29 whole cell lysate
3. 10 µg of HT-29 whole cell lysate (input)
