ARG11027
anti-Cytokeratin 5 + 6 antibody [T16-K]
anti-Cytokeratin 5 + 6 antibody [T16-K] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Rabbit Monoclonal antibody [T16-K] recognizes Cytokeratin 5 + 6 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 特异性 | ARG11027 anti-Cytokeratin 5 + 6 antibody [T16-K] recognizes the epitope between Thr548 - His564 (identical with Cytokeratin 5 and 6). |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | T16-K |
| 靶点名称 | Cytokeratin 5 + 6 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human Cytokeratin 6. |
| 抗原表位 | Between Thr548 - His564 (identical with Cytokeratin 5 and 6). |
| 偶联标记 | Un-conjugated |
| 別名 | K5; CK5; DDD; DDD1; EBS2; KRT5A; CK-6A; K6D; Cytokeratin-6A; CK-6D; K6C; K6A; Type-II keratin Kb6; PC3; CK6D; KRT6D; CK6A; CK6C; allergen Hom s 5; Keratin, type II cytoskeletal 6A; Keratin-6A; Cytokeratin-6D; KRT6C |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section (95 - 97°C) in Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0) for 25 min followed by cooling at RT for 15 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human skin or prostate tissue (basal cells); squamous cell carcinoma tissue. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT5; KRT6A |
| 全名 | keratin 5; keratin 6A, type II |
| 背景介绍 | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014] |
| 生物功能 | Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. [UniProt] |
| 细胞定位 | Cytoplasm |
| 预测分子量 | 60 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG11027 anti-Cytokeratin 5 + 6 antibody [T16-K] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsillary epithelium (4 µm sections) stained with ARG11027 anti-Cytokeratin 5 + 6 antibody [T16-K].
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ARG11027 anti-Cytokeratin 5 + 6 antibody [T16-K] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human epidermis of the skin (4 µm sections) stained with ARG11027 anti-Cytokeratin 5 + 6 antibody [T16-K].
