ARG62981
anti-Cytokeratin 7 + 17 antibody [C-46]
anti-Cytokeratin 7 + 17 antibody [C-46] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Bovine,Pig
CK7/CK20 Carcinoma Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [C-46] recognizes Cytokeratin 7 + 17 |
|---|---|
| 反应物种 | Hu, Bov, Pig |
| 不反应物种 | Ms, Rat, Rb |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | The clone C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | C-46 |
| 同位型 | IgG1 |
| 靶点名称 | Cytokeratin 7 + 17 |
| 抗原物种 | Human |
| 抗原 | Cytoskeleton preparation of HeLa human cervix carcinoma cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing condition). IHC-P: Pretreatment: 0.1% pepsin for 30 min at 37°C. Incubation: 4°C overnight. Application note: Chromogen DAB. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: HeLa IHC-P: Pancreas |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT7 |
| 全名 | keratin 7, type II |
| 背景介绍 | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| 研究领域 | CK7/CK20 Carcinoma Study antibody |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. |
检测图片 (3) Click the Picture to Zoom In
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ARG62981 anti-Cytokeratin 7 + 17 antibody [C-46] WB image
Western blot: HeLa cell lysate stained with ARG62981 anti-Cytokeratin 7 + 17 antibody [C-46].
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ARG62981 anti-Cytokeratin 7 + 17 antibody [C-46] IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue stained with ARG62981 anti-Cytokeratin 7 + 17 antibody [C-46].
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ARG62981 anti-Cytokeratin 7 + 17 antibody [C-46] IHC-P image
Immunohistochemistry: Pancreas (paraffin-embedded sections) stained with ARG62981 anti-Cytokeratin 7 + 17 antibody [C-46].
克隆号文献
Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis.
A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
