ARG62463
anti-Cytokeratin 7 antibody [OV-TL 12/30]
anti-Cytokeratin 7 antibody [OV-TL 12/30] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
CK7/CK20 Carcinoma Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [OV-TL 12/30] recognizes Cytokeratin 7 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | This antibody reacts with the 54 kD cytokeratin 7 intermediate filament protein |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | OV-TL 12/30 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Cytokeratin 7 |
| 抗原物种 | Human |
| 抗原 | OTN 11, ovarian carcinoma cell line |
| 偶联标记 | Un-conjugated |
| 別名 | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 20 mM tris-borate (pH 7.5), 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM, fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 稳定剂 | fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT7 |
| 全名 | keratin 7, type II |
| 背景介绍 | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | CK7/CK20 Carcinoma Study antibody |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. |
检测图片 (1) Click the Picture to Zoom In
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ARG62463 anti-Cytokeratin 7 antibody [OV-TL 12/30] WB image
Western blot: 30 µg of A549 cell lysate stained with ARG62463 anti-Cytokeratin 7 antibody [OV-TL 12/30] at 1:50 dilution.
客户反馈
Excellent
anti-Cytokeratin 7 antibody [OV-TL 12/30]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
克隆号文献
Serrated pathway adenocarcinomas: molecular and immunohistochemical insights into their recognition.
IHC / Human
Mammalian target of rapamycin signalling modulates amino acid uptake by regulating transporter cell surface abundance in primary human trophoblast cells.
WB / Human
Derivation and characterization of matched cell lines from primary and recurrent serous ovarian cancer.
WB / Human
Immunohistochemical comparative study of fibrosis and biliary ductular reaction in alcoholic and viral chronic hepatitis.
IHC / Human
Fusion of villous trophoblast can be visualized by localizing active caspase 8.
ICC/IF / Human
