ARG66340
anti-Cytokeratin 7 antibody [SQab1888]
anti-Cytokeratin 7 antibody [SQab1888] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
CK7/CK20 Carcinoma Study antibody
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1888] recognizes Cytokeratin 7 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1888 |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 7 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 1-100 of Cytokeratin 7. |
| 偶联标记 | Un-conjugated |
| 別名 | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT7 |
| 全名 | keratin 7, type II |
| 背景介绍 | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30318 CK7 / CK20 Carcinoma Antibody Duo Related products: Cytokeratin 7 antibodies; Cytokeratin 7 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer |
| 研究领域 | CK7/CK20 Carcinoma Study antibody |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IHC-P image
Immunohistochemistry: 10% Formalin-fixed and paraffin-embedded Mouse tumor tissue. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:3000 dilution for 30 min at 37ºC.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] (green) at 1:2,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human thyroid cancer tissue. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:3200 dilution.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] WB image
Western blot: 10 µg of HeLa, A549 and HaCat cell lysates stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888] at 1:10000 dilution.
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ARG66340 anti-Cytokeratin 7 antibody [SQab1888] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66340 anti-Cytokeratin 7 antibody [SQab1888]. 1) ARG66340 IP in HeLa whole cell lysate, 2) PBS instead of ARG66340 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 10 µg (input).
客户反馈
Excellent
anti-Cytokeratin 7 antibody [SQab1888]
Application：IHC
Sample：Mouse tumor
Sample Preparation Method：Paraffin-embedded
Fixation Buffer：10% Formalin
Antigen Retrieval Buffer：Tris/EDTA buffer (pH 9.0)
Antigen Retrieval Temperature：98 ºC
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：30 min
Primary Antibody Incubation Temperature：37 ºC
Excellent
anti-Cytokeratin 7 antibody [SQab1888]
Application：IHC
Sample：Mouse tumor
Sample Preparation Method：Paraffin-embedded
Fixation Buffer：10% Formalin
Antigen Retrieval Buffer：Tris/EDTA buffer (pH 9.0)
Antigen Retrieval Temperature：98 ºC
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：30 min
Primary Antibody Incubation Temperature：37 ºC
