ARG66815

Name: anti-Cytokeratin 8 + 18 antibody [SQab20229]
Brand: arigo Biolaboratories
SKU: ARG66815
Price: 399 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-Cytokeratin 8 + 18 antibody [SQab20229]

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab20229] recognizes Cytokeratin 8 + 18
反应物种	Hu
应用	IHC-P, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab20229
同位型	IgG
靶点名称	Cytokeratin 8 + 18
抗原物种	Human
抗原	Synthetic peptide within aa. 383 to C-terminal of Human Cytokeratin 8 and synthetic peptide within aa. 330-430 of Human Cytokeratin 18.
偶联标记	Un-conjugated
別名	KRT8: Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 KRT18: Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

应用说明

应用建议

应用	推荐稀释比
IHC-P	1:100 - 1:200
WB	1:500-1:2000

应用说明

IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	KRT8; KRT18
全名	keratin 8, type II; keratin 18, type I
背景介绍	Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能	Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
细胞定位	KRT8: Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. [UniProt]
预测分子量	Cytokeratin 8: 54 kDa; Cytokeratin 18: 48 kDa
翻译后修饰	KRT8: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt] KRT18: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt]

检测图片 (2) Click the Picture to Zoom In

ARG66815 anti-Cytokeratin 8 + 18 antibody [SQab20229] IHC-P image

Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human colon tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66815 anti-Cytokeratin 8 + 18 antibody [SQab20229].
ARG66815 anti-Cytokeratin 8 + 18 antibody [SQab20229] WB image (Customer review)

Western blot: HeLa stained with ARG66815 anti-Cytokeratin 8 + 18 antibody [SQab20229] at 1:1000 dilution.