ARG56044
anti-Cytokeratin 8 antibody [B22.1]
anti-Cytokeratin 8 antibody [B22.1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [B22.1] recognizes Cytokeratin 8 |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat, Chk, Xenopus laevis |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | B22.1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Cytokeratin 8 |
| 抗原物种 | Human |
| 抗原 | A cytoskeletal preparation from HeLa cells. |
| 偶联标记 | Un-conjugated |
| 別名 | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | HCT-116 | ||||||||||
| 实际分子量 | ~ 46 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT8 |
| 全名 | keratin 8, type II |
| 背景介绍 | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
检测图片 (3) Click the Picture to Zoom In
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ARG56044 anti-Cytokeratin 8 antibody [B22.1] ICC/IF image
Immunofluorescence: Permeabilized MCF7 cells stained with ARG56044 anti-Cytokeratin 8 antibody [B22.1] (green). Reddot (red) for nuclear staining.
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ARG56044 anti-Cytokeratin 8 antibody [B22.1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded colon carcinoma stained with ARG56044 anti-Cytokeratin 8 antibody [B22.1].
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ARG56044 anti-Cytokeratin 8 antibody [B22.1] WB image
Western blot: HCT-116 cell lysate stained with ARG56044 anti-Cytokeratin 8 antibody [B22.1].
