ARG62983
anti-Cytokeratin 8 antibody [C-43]
anti-Cytokeratin 8 antibody [C-43] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Bovine,Pig,Rabbit,Sheep
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [C-43] recognizes Cytokeratin 8 |
|---|---|
| 反应物种 | Hu, Bov, Pig, Rb, Sheep |
| 不反应物种 | Ms, Rat, Chk, Hm, Xenopus laevis |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | The clone C-43 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | C-43 |
| 同位型 | IgG1 |
| 靶点名称 | Cytokeratin 8 |
| 抗原物种 | Human |
| 抗原 | Cytoskeleton preparation from HeLa human cervix carcinoma cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | IHC-P: Prostate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT8 |
| 全名 | keratin 8, type II |
| 背景介绍 | Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. |
| 生物功能 | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
检测图片 (2) Click the Picture to Zoom In
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ARG62983 anti-Cytokeratin 8 antibody [C-43] IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue stained with ARG62983 anti-Cytokeratin 8 antibody [C-43].
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ARG62983 anti-Cytokeratin 8 antibody [C-43] WB image
Western blot: HeLa cell lysate stained with ARG62983 anti-Cytokeratin 8 antibody [C-43], in reducing conditions.
克隆号文献
CARM1 and PRMT1 are dysregulated in lung cancer without hierarchical features.
WB / Human
Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation.
ICC/IF / Sheep
Mechanistic insight into taxol-induced cell death.
WB / Human
