ARG70024
anti-Cytokeratin 8 antibody (PE)
anti-Cytokeratin 8 antibody (PE) for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | RPE-conjugated Rabbit Polyclonal antibody recognizes Cytokeratin 8 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 8 |
| 抗原物种 | Human |
| 抗原 | Recombinint protein around aa. 1-483 of Human Cytokeratin 8. |
| 偶联标记 | PE |
| 別名 | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Powder |
|---|---|
| 纯化 | Affinity purified. |
| 复溶 | Reconstitute the antibody with 0.4 ml of distilled water to produce a 75 μg/ml solution. Allow the antibody to sit for 10 minutes with gentle agitation prior to use.Use the antibody within 30 days of reconstitution. |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT8 |
| 全名 | keratin 8, type II |
| 背景介绍 | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG70024 anti-Cytokeratin 8 antibody (PE) ICC/IF image
Immunofluorescence: HeLa cells stained with ARG70024 anti-Cytokeratin 8 antibody (PE) at 5 µg/ml dilution.
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ARG70024 anti-Cytokeratin 8 antibody (PE) FACS image
Flow Cytometry: A549 cells unstained (left), stained with isotype control antibody (middle) or ARG70024 anti-Cytokeratin 8 antibody (PE) (right).
