ARG51782
anti-Cytokeratin 8 phospho (Ser74) antibody
anti-Cytokeratin 8 phospho (Ser74) antibody for ICC/IF,Western blot and Human
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cytokeratin 8 phospho (Ser74) |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 8 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of Ser74 (L-L-S(p)-P-L) derived from Human Cytokeratin 8 (CK8). |
| 偶联标记 | Un-conjugated |
| 別名 | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT8 |
| 全名 | keratin 8, type II |
| 背景介绍 | Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. |
| 生物功能 | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
检测图片 (3) Click the Picture to Zoom In
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ARG51782 anti-Cytokeratin 8 phospho (Ser74) antibody WB image
Western blot: Extracts from HepG2 cells untreated or treated with EGF and stained with ARG51782 anti-Cytokeratin 8 phospho (Ser74) antibody.
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ARG51782 anti-Cytokeratin 8 phospho (Ser74) antibody WB image
Western blot: Extracts from 293 cells, treated with calf intestinal phosphatase (CIP), stained with ARG51782 anti-Cytokeratin 8 phospho (Ser74) antibody.
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ARG51782 anti-Cytokeratin 8 phospho (Ser74) antibody ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51782 anti-Cytokeratin 8 phospho (Ser74) antibody.
