ARG56128

anti-Cytokeratin (pan) antibody [PAN-CK]

anti-Cytokeratin (pan) antibody [PAN-CK] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

publication_link 参考文献1

概述

产品描述 Mouse Monoclonal antibody [PAN-CK] recognizes Cytokeratin (pan)
反应物种 Hu, Ms, Rat
应用 FACS, IHC-P
特异性 Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK7, CK8, CK10, CK13, CK14, CK15, CK16, CK17, CK18 and CK19.
宿主 Mouse
克隆 Monoclonal
克隆号 PAN-CK
同位型 IgG, kappa
靶点名称 Cytokeratin (pan)
抗原物种 Human
抗原 Human epidermal keratin
偶联标记 Un-conjugated
別名 Type-II keratin Kb39; KRT1B; Keratin-77; K1B; K77; CK-1B; Cytokeratin-1B; Keratin, type II cytoskeletal 1b

应用说明

应用建议
应用 推荐稀释比
FACS0.5 - 1 µg/10^6 cells
IHC-P0.5 - 1 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 374454 Human KRT77

GeneID: 406220 Mouse KRT77

GeneID: 406226 Rat KRT77

基因名称 KRT77
全名 keratin 77, type II
背景介绍 Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]
预测分子量 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 55 kDa (CK7); 52 kDa (CK8); 56.5 kDa (CK10); 53 kDa (CK13); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 46 kDa (CK17); 45 kDa (CK18) and 40 kDa (CK19).
翻译后修饰 Undergoes deimination of some arginine residues (citrullination).

检测图片 (1) Click the Picture to Zoom In

  • ARG56128 anti-Cytokeratin (pan) antibody [PAN-CK] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human skin stained with ARG56128 anti-Cytokeratin (pan) antibody [PAN-CK].

文献引用

Long-term remission under Disitamab Vedotin (RC48) in HR-positive/HER2-positive metastatic breast cancer with brain meningeal, and bone marrow involvement: A case report

IHC-P / Human

Qifeng Wu et al.
Oncol Lett.,  (2022)

publication_link

 

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