ARG43800
anti-DCT / TRP2 antibody
anti-DCT / TRP2 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DCT / TRP2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DCT / TRP2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to aa. Q24-A519 of Human DCT / TRP2. |
| 偶联标记 | Un-conjugated |
| Protein Full name | L-dopachrome tautomerase |
| 別名 | L-dopachrome tautomerase; TYRP2; Tyrosinase-related protein 2; L-dopachrome Delta-isomerase; EC 5.3.3.12; TRP2; DCT; DT; TRP-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | rat testis tissue; C6 cell line; mouse testis; RAW264.7 cell line | ||||||
| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DCT |
| 全名 | dopachrome tautomerase |
| 生物功能 | Involved in regulating eumelanin and phaeomelanin levels. [UniProt] |
| 细胞定位 | Melanosome membrane; Single-pass type I membrane protein. Melanosome. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. [UniProt] |
| 预测分子量 | Isoform 1: 59 kDa Isoform 2: 63 kDa |
| 翻译后修饰 | Glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG43800 anti-DCT / TRP2 antibody FACS image
Flow Cytometry: MCF-7 cells were blocked with 10% normal goat serum and then stained with ARG43800 anti-DCT / TRP2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43800 anti-DCT / TRP2 antibody WB image
Western blot: rat testis tissue lysates, rat C6 whole cell lysates, mouse testis tissue lysates, mouse RAW264.7 cell lysates stained with ARG43800 anti-DCT / TRP2 antibody at 1:1000 dilution.
