ARG54096
anti-DLAT antibody
anti-DLAT antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes DLAT |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | DLAT |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human DLAT protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; PDC-E2; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; EC 2.3.1.12; PBC; Pyruvate dehydrogenase complex component E2; DLTA; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PDCE2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P10515 Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydr Swiss-port # Q8BMF4 Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydr |
|---|---|
| 基因名称 | DLAT |
| 全名 | dihydrolipoamide S-acetyltransferase |
| 背景介绍 | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). |
| 生物功能 | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt] |
| 细胞定位 | Mitochondrion matrix. |
| 研究领域 | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Delipoylated at Lys-132 and Lys-259 by SIRT4, delipoylation decreases the PHD complex activity. |
检测图片 (3) Click the Picture to Zoom In
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ARG54096 anti-DLAT antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54096 anti-DLAT antibody at 1:300 dilution.
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ARG54096 anti-DLAT antibody WB image
Western blot: Jurkat cell lysate stained with ARG54096 anti-DLAT antibody at 1:1000 dilution.
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ARG54096 anti-DLAT antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54096 anti-DLAT antibody.
