ARG65660
anti-Dengue virus NS1 antibody [SQab1501]
anti-Dengue virus NS1 antibody [SQab1501] for ELISA,ICC/IF,Western blot and Dengue virus
Microbiology and Infectious Disease antibody
参考文献6
概述
| 产品描述 | Mouse Monoclonal antibody [SQab1501] recognizes Dengue virus NS1 |
|---|---|
| 反应物种 | DEN |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | This antibody recognizes monomer, dimer, hexamer of DENV1/2/3/4 NS1 protein. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SQab1501 |
| 同位型 | IgG2a |
| 靶点名称 | Dengue virus NS1 |
| 抗原物种 | Virus |
| 抗原 | Recombinant hexamer Dengue virus NS1 protein from drosophila cell |
| 偶联标记 | Un-conjugated |
| 別名 | Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS (pH 7.4), 0.01% Thimerosal, 1% BSA and 10% Glycerol. |
| 抗菌剂 | 0.01% Thimerosal |
| 稳定剂 | 1% BSA and 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | DENV_gp1 |
|---|---|
| 全名 | Dengue virus nonstructural protein 1 |
| 背景介绍 | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| 生物功能 | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| 产品亮点 | Related products: Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New Dengue Virus NS1 antibodies and Antibody Duo Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Tools for studying Dengue Virus Exploring Antiviral Immune Response |
| 研究领域 | Microbiology and Infectious Disease antibody |
检测图片 (3) Click the Picture to Zoom In
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ARG65660 anti-Dengue virus NS1 antibody [SQab1501] ICC/IF image
Immunofluorescence: ARG65660 anti-Dengue virus NS1 antibody [SQab1501] (1:400) were used for detecting DENV NS1.
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ARG65660 anti-Dengue virus NS1 antibody [SQab1501] WB image
Western blot: 6 μg of Vero cells Infected with 1) Mock, 2) ZIKV, 3) DENV1, 4) DENV2, 5) DENV3 and 6) DENV 4. Cell lysates were stained with ARG65660 anti-Dengue virus NS1 antibody [SQab1501] at 1:2000.
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ARG65660 anti-Dengue virus NS1 antibody [SQab1501] WB image
Western blot: 6 μg of DENV2-infected C636 and ZIKV-infected C636 cell lysates stained with ARG65660 anti-Dengue virus NS1 antibody [SQab1501] at 0.5 μg/ml dilution.
客户反馈
Excellent
anti-Dengue virus NS1 antibody [SQab1501]
Application：FACS
Sample：Dengue virus type 2 (strain 16681) infected monocyte cells (M.O.I.=10)
Cell Number/Concentration：1x10E6
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：1hr
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：AlexaFluor488
文献引用
SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication
WB / Dengue virus
NS1 Protein N-Linked Glycosylation Site Affects the Virulence and Pathogenesis of Dengue Virus
WB / Dengue Virus
N-Butanol Extract of Glycyrrhizae Radix et Rhizoma Inhibits Dengue Virus through Targeting Envelope Protein
WB, ICC/IF / Dengue virus
A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response
WB / Dengue virus
DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junction.
WB, ICC/IF / Dengue virus
MicroRNA-155 inhibits dengue virus replication by inducing heme oxygenase-1-mediated antiviral interferon responses.
IHC-P / Dengue virus
