ARG43768
anti-E Cadherin phospho (Ser838 / Ser840) antibody
anti-E Cadherin phospho (Ser838 / Ser840) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
EMT Study antibody; Epithelial Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes E Cadherin phospho (Ser838 / Ser840). |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | E Cadherin |
| 抗原物种 | Human |
| 抗原 | Synthetic phosphopeptide corresponding to residues surrounding Ser838/Ser840 of human E‐Cadherin. |
| 偶联标记 | Un-conjugated |
| 別名 | Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324 |
应用说明
| 应用建议 |
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| 应用说明 | IHC: Antigen retrieval: Heat mediated was performed using Citrate buffer pH 6.0 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDH1 |
| 全名 | cadherin 1, type 1 |
| 背景介绍 | E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| 生物功能 | Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30320 EMT Marker Antibody Panel Related products: E Cadherin antibodies; E Cadherin ELISA Kits; E Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) New EMT antibody panel is released |
| 研究领域 | EMT Study antibody; Epithelial Marker antibody |
| 预测分子量 | 97 kDa |
| 翻译后修饰 | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. |
检测图片 (2) Click the Picture to Zoom In
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ARG43768 anti-E Cadherin phospho (Ser838 / Ser840) antibody IHC-P image
Immunohistochemistry: Human breast cancer tissue stained with ARG43768 anti-E Cadherin phospho (Ser838 / Ser840) antibody. Antigen retrieval: heat the tissue in citrate buffer (pH6.0).
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ARG43768 anti-E Cadherin phospho (Ser838 / Ser840) antibody WB image
Western blot: HepG2, U2OS and U251 cell lysate stained with ARG43768 anti-E Cadherin phospho (Ser838 / Ser840) antibody.
