ARG57945
anti-ETV4 / Pea3 antibody
anti-ETV4 / Pea3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ETV4 / Pea3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ETV4 / Pea3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-207 of Human ETV4 (NP_001248368.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Polyomavirus enhancer activator 3 homolog; Adenovirus E1A enhancer-binding protein; PEAS3; PEA3; Protein PEA3; E1AF; E1A-F; ETS translocation variant 4 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | H460 | ||||||||
| 实际分子量 | 55 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ETV4 |
| 全名 | ets variant 4 |
| 生物功能 | Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG57945 anti-ETV4 / Pea3 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG57945 anti-ETV4 / Pea3 antibody.
-
ARG57945 anti-ETV4 / Pea3 antibody WB image
Western blot: 25 µg of H460 cell lysate stained with ARG57945 anti-ETV4 / Pea3 antibody at 1:1000 dilution.
