ARG54703

Name: anti-EZH2 antibody
Brand: arigo Biolaboratories
SKU: ARG54703
Price: 399 USD
Availability: InStock
Rating: 4.5 (4 reviews)

anti-EZH2 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399
258

50 μl

279
181

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes EZH2
反应物种	Hu, Ms
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	EZH2
抗原物种	Human
抗原	Human EZH2 recombinant protein (NP_001190176.1).
偶联标记	Un-conjugated
別名	ENX-1; Lysine N-methyltransferase 6; KMT6; ENX1; WVS2; WVS; Enhancer of zeste homolog 2; EZH2b; Histone-lysine N-methyltransferase EZH2; EZH1; EC 2.1.1.43; KMT6A

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
ICC/IF	1:10 - 1:50
IHC-P	1:10 - 1:50
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

纯化	Protein A purified
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 14056 Mouse EZH2 GeneID: 2146 Human EZH2 Swiss-port # Q15910 Human Histone-lysine N-methyltransferase EZH2 Swiss-port # Q61188 Mouse Histone-lysine N-methyltransferase EZH2
基因名称	EZH2
全名	enhancer of zeste 2 polycomb repressive complex 2 subunit
背景介绍	This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
生物功能	Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. [From Uniprot]
细胞定位	Nucleus
产品亮点	Related Antibody Duos and Panels: ARG30309 Polycomb Complexes Antibody Panel (H3K27me3, EZH2, Ring1A, RNF2) Related products: EZH2 antibodies; EZH2 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域	Cancer antibody; Gene Regulation antibody; Polycomb Complexes antibody
预测分子量	85 kDa
翻译后修饰	Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.

检测图片 (5) Click the Picture to Zoom In

ARG54703 anti-EZH2 antibody WB image

Western blot: 30 µg of U2OS, Jurkat and PC3 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution
ARG54703 anti-EZH2 antibody WB image

Western blot: 30 µg of SW480 and HCT116 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution.
ARG54703 anti-EZH2 antibody ICC/IF image

Immunofluorescence: 293 cells stained with ARG54703 anti-EZH2 antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
ARG54703 anti-EZH2 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG54703 anti-EZH2 antibody.
ARG54703 anti-EZH2 antibody FACS image

Flow Cytometry: HeLa cells stained with ARG54703 anti-EZH2 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.