ARG44656

anti-Enolase 1 antibody

anti-Enolase 1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

概述

产品描述 Mouse Monoclonal antibody recognizes Enolase 1
反应物种 Hu
应用 ELISA, IHC-P, IP, WB
宿主 Mouse
克隆 Monoclonal
同位型 IgG1
靶点名称 Enolase 1
抗原物种 Human
偶联标记 Un-conjugated
別名 ENO1; Enolase 1; Alpha-Enolase; ENO1L1; MBP-1; MPB1; PPH; ENO1 Intronic Transcript 1 (Non-Protein Coding); 2-Phospho-D-Glycerate Hydro-Lyase; Plasminogen-Binding Protein; ENO1 Intronic Transcript 1; Phosphopyruvate Hydratase; Enolase 1, (Alpha); Non-Neural Enolase; EC 4.2.1.11; ENO1-IT1; NNE; Epididymis Secretory Protein Li 17; C-Myc Promoter-Binding Protein-1; MYC Promoter-Binding Protein 1; C-Myc Promoter-Binding Protein; Alpha Enolase Like 1; Tau-Crystallin; Enolase-Alpha; HEL-S-17; EC 4.2.1; MBPB1; MPB-1

应用说明

应用建议
应用 推荐稀释比
ELISA5 µg/mL
IHC-P2 µg/mL
IP10 µg/mL
WB1 µg/mL
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Protein A purification
缓冲液 PBS with 0.09% sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 2023 Human ENO1

Swiss-port # P06733 Human Alpha-enolase

基因名称 ENO1
全名 Enolase 1
背景介绍 This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
生物功能 Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate.

Stimulates immunoglobulin production. [UniProt]
细胞定位 Cell membrane, Cytoplasm, Membrane, Nucleus. [UniProt]
预测分子量 47 kDa
翻译后修饰 Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG44656 anti-Enolase 1 antibody IHC-P image

    Immunohistochemistry: Human Colon stained with ARG44656 anti-Enolase 1 antibody at 2 µg/mL dilution.

  • ARG44656 anti-Enolase 1 antibody WB image

    Western blot: SH-SY5Y, U-251MG, MCF-7, A-431, HeLa, THP-1, Jurkat stained with ARG44656 anti-Enolase 1 antibody at 1 µg/mL dilution.

  • ARG44656 anti-Enolase 1 antibody IP image

    Immunoprecipitation: MCF7 lysate immunoprecipitated with 2.5 µg of ARG44656 anti-Enolase 1 antibody.