ARG62815
anti-EpCAM antibody [323/A3]
anti-EpCAM antibody [323/A3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
概述
| 产品描述 | Mouse Monoclonal antibody [323/A3] recognizes CD326 / EpCAM |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Rat |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | The clone 323/A3 recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 323/A3 |
| 同位型 | IgG1 |
| 靶点名称 | EpCAM |
| 抗原物种 | Human |
| 抗原 | Human breast cancer MCF-7 cells_x000D_ |
| 偶联标记 | Un-conjugated |
| 別名 | MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: A431, MCF7 and SW480 IHC-P: Breast carcinoma |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EPCAM |
| 全名 | epithelial cell adhesion molecule |
| 背景介绍 | EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008] |
| 生物功能 | EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt] |
| 研究领域 | Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
检测图片 (2) Click the Picture to Zoom In
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ARG62815 anti-EpCAM antibody [323/A3] WB image
Western blot: MCF7 whole cell lysate stained with ARG62815 anti-EpCAM antibody [323/A3], in non-reducing conditions.
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ARG62815 anti-EpCAM antibody [323/A3] WB image
Western blot: MCF7 whole cell lysate stained with ARG62815 anti-EpCAM antibody [323/A3], under non-reducing condition.
克隆号文献
The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer.
WB / Human
Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.
ICC/IF / Human
Further evidence for EpCAM as the gene for congenital tufting enteropathy.
IHC-P / Human
Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy.
IHC / Human
Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers.
FACS / Mouse
