ARG66353

Name: anti-EpCAM antibody [SQab18103]
Brand: arigo Biolaboratories
SKU: ARG66353
Price: 399 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-EpCAM antibody [SQab18103]

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab18103] recognizes EpCAM
反应物种	Hu
应用	IHC-P, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab18103
同位型	IgG
靶点名称	EpCAM
抗原物种	Human
抗原	Synthetic peptide within aa. 1-100 of Human EpCAM.
偶联标记	Un-conjugated
別名	MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

应用说明

应用建议

应用	推荐稀释比
IHC-P	1:100 - 1:200
WB	1:500 - 1:4000

应用说明

IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

35 kDa

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4072 Human EPCAM Swiss-port # P16422 Human Epithelial cell adhesion molecule
基因名称	EPCAM
全名	epithelial cell adhesion molecule
背景介绍	EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
生物功能	EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
产品亮点	Related products: EpCAM antibodies; EpCAM ELISA Kits; EpCAM Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones)
研究领域	Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
预测分子量	35 kDa
翻译后修饰	Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]

检测图片 (2) Click the Picture to Zoom In

ARG66353 anti-EpCAM antibody [SQab18103] WB image (Customer's Feedback)

Western blot: HT-29 cell lysate stained with ARG66353 anti-EpCAM antibody [SQab18103] at 1:1000 dilution.
ARG66353 anti-EpCAM antibody [SQab18103] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded colon tissue stained with ARG66353 anti-EpCAM antibody [SQab18103]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).