ARG65617
anti-FADD antibody
anti-FADD antibody for Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
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概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FADD. |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FADD |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human FADD (NP_003815.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Mediator of receptor induced toxicity; MORT1; GIG3; FAS-associated death domain protein; Growth-inhibiting gene 3 protein; Protein FADD; FAS-associating death domain-containing protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13158 Human FAS-associated death domain protein Swiss-port # Q61160 Mouse FAS-associated death domain protein |
|---|---|
| 基因名称 | FADD |
| 全名 | Fas (TNFRSF6)-associated via death domain |
| 背景介绍 | The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008] |
| 生物功能 | Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt] |
| 产品亮点 | Related products: FADD antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 23 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65617 anti-FADD antibody WB image
Western blot: HeLa cell lysate stained with ARG65617 anti-FADD antibody.
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ARG65617 anti-FADD antibody IP image
Immunoprecipitation: 200 µg extracts of HeLa cells were immunoprecipitated and stained with ARG65617 anti-FADD antibody at 1:1000 dilution.
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anti-FADD antibody
Application：WB
Sample：293T and HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
