ARG57088
anti-FLIP antibody [8B12]
anti-FLIP antibody [8B12] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [8B12] recognizes FLIP |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 8B12 |
| 同位型 | IgG1, kappa |
| 靶点名称 | FLIP |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-480 of Human FLIP. |
| 偶联标记 | Un-conjugated |
| 別名 | FLAME1; CASP8AP1; c-FLIPL; FLIP; c-FLIPR; c-FLIPS; MACH-related inducer of toxicity; Inhibitor of FLICE; c-FLIP; CASH; Cellular FLICE-like inhibitory protein; CASP8 and FADD-like apoptosis regulator; Caspase homolog; FLAME; FADD-like antiapoptotic molecule 1; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; I-FLICE; FLAME-1; MRIT; Casper; Usurpin; CLARP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O15519 Human CASP8 and FADD-like apoptosis regulator |
|---|---|
| 基因名称 | CFLAR |
| 全名 | CASP8 and FADD-like apoptosis regulator |
| 背景介绍 | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011] |
| 生物功能 | Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. [UniProt] |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. |
检测图片 (2) Click the Picture to Zoom In
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ARG57088 anti-FLIP antibody [8B12] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57088 anti-FLIP antibody [8B12] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57088 anti-FLIP antibody [8B12] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) HeLa cell lysate, 3) MCF7 cell lysate, 4) K562 cell lysate stained with ARG57088 anti-FLIP antibody [8B12] at 1:1000.
