ARG10712
anti-FOX3 / NeuN antibody
anti-FOX3 / NeuN antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FOX3 / NeuN |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Hrs, Pig |
| 预测物种 | Chk |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FOX3 / NeuN |
| 抗原物种 | Human |
| 抗原 | N-terminal 100 aa. of Human Fox3 expressed in and purified from E. coli. |
| 偶联标记 | Un-conjugated |
| 別名 | RNA binding protein fox-1 homolog 3; NEUN; FOX-3; HRNBP3; Fox-1 homolog C; FOX3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # A6NFN3 Human RNA binding protein fox-1 homolog 3 Swiss-port # Q8BIF2 Mouse RNA binding protein fox-1 homolog 3 |
|---|---|
| 基因名称 | RBFOX3 |
| 全名 | RNA binding protein, fox-1 homolog (C. elegans) 3 |
| 生物功能 | RNA-binding protein that regulates alternative splicing events. [UniProt] |
| 预测分子量 | 34 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG10712 anti-FOX3 / NeuN antibody WB image
Western blot: 30 µg of U87, Mouse brain and Rat brain lysates stained with ARG10712 anti-FOX3 / NeuN antibody at 1:5000 dilution.
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ARG10712 anti-FOX3 / NeuN antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse C57BL/6Jnarl brain tissue. The tissue section was fixed by 4% formalin and blocked with BSA with 3% Goat serum, at RT for 1 hour. Tissue section was then stained with ARG10712 anti-FOX3 / NeuN antibody at 1:500 dilution, in PBS with 1% Goat serum, overnight at 4°C.
Blue: DAPI
Yellow: Venus reporter gene
Red: NueN -
ARG10712 anti-FOX3 / NeuN antibody IHC-Fr image
Immunohistochemistry: Paraformaldehyde-fixed frozen section of adult Rat brain stem stained with ARG10712 anti-FOX3 / NeuN antibody (red) and co-stained for MAP2 (green). DNA is shown in blue. Note that RPCA-Fox3 stains neuronal nuclei and distal perikarya and that the MAP2 antibody stains the dendrites extending from these cells.
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ARG10712 anti-FOX3 / NeuN antibody IHC-Fr image
Immunohistochemistry: Frozen section of Adult Mouse cerebellum stained with ARG10712 anti-FOX3 / NeuN antibody (red) at 1:5000 dilution and costained with ARG52313 anti-GFAP antibody (green) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours and 45 µm free-floating sections were stained with above antibodies.)
The FOX3 / NeuN antibody stains the nuclei of neurons in the cerebellar granule layer. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granule and white matter layers.
客户反馈
Good
anti-FOX3 / NeuN antibody
Application：IHC
Sample：Mouse C57BL/6Jnarl brain tissue.
Sample Preparation Method：Frozen
Fixation Buffer：4% formalin
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：Dylight 594
Good
anti-FOX3 / NeuN antibody
Application：WB
Sample：U87, Mouse brain and Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques
IHC-Fr / Human
