ARG63332

anti-FOXC2 antibody

anti-FOXC2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Chondrogenesis Study antibody
publication_link 参考文献1

概述

产品描述 Goat Polyclonal antibody recognizes FOXC2
反应物种 Hu
预测物种 Cow, Rat, Dog
应用 FACS, IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 FOXC2
抗原物种 Human
抗原 RHAAPYSYDCTKY
偶联标记 Un-conjugated
別名 LD; FKHL14; Transcription factor FKH-14; Forkhead box protein C2; MFH-1; MFH1; MFH-1 protein; Mesenchyme fork head protein 1; Forkhead-related protein FKHL14

应用说明

应用建议
应用 推荐稀释比
FACS10 µg/ml
IHC-PAssay-dependent
WB1 - 2 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 2303 Human FOXC2

Swiss-port # Q99958 Human Forkhead box protein C2

背景介绍 This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]
产品亮点 Related Antibody Duos and Panels:
ARG30314 Chondrogenesis Marker Antibody Panel
Related products:
FOXC2 antibodies; FOXC2 Duos / Panels; Anti-Goat IgG secondary antibodies;
研究领域 Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Chondrogenesis Study antibody
预测分子量 54 kDa
翻译后修饰 Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin.

检测图片 (2) Click the Picture to Zoom In

  • ARG63332 anti-FOXC2 antibody WB image

    Western blot: 35 µg of HEK293 nucleus (A) and Human pancreas (B, negative control) lysates (in RIPA buffer) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.

  • ARG63332 anti-FOXC2 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63332 anti-FOXC2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

文献引用

Expression and clinical significance of FOXC2 and E-cad in colon cancer tissues (FOXC2和E-cad在结肠癌组织中的表达及临床意义)

IHC-P / Human

Wang Jing-Miao (王静苗) et al.
Chinese Clinical Oncology (临床肿瘤学杂志), 21(8),  (2016)

publication_link

 

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