ARG54202
anti-FOXP3 antibody [3G3]
anti-FOXP3 antibody [3G3] for Flow cytometry,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [3G3] recognizes FOXP3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, WB |
| 特异性 | The mouse monoclonal antibody 3G3 recognizes Nterminal region of FoxP3, a 4755 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3G3 |
| 同位型 | IgG1 |
| 靶点名称 | FOXP3 |
| 抗原物种 | Mouse |
| 抗原 | Full-length His-tagged recombinant murine FoxP3 |
| 偶联标记 | Un-conjugated |
| 別名 | FOXP3; Forkhead Box P3; DIETER; XPID; AIID; PIDX; IPEX; JM2; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; SCURFIN; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; FOXP3delta7; Scurfin |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Staining method: - Perform staining of cell surface markers (CD25, CD4 etc.) for 10 min. at 4°C in the dark; 1 million cells in 100 µl final volume. - Add 1-2 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. - Resuspend the cells in 1 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 1-2 ml of ice-cold PBS with 1% BSA. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 1-2 ml of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 80µl of ice-cold permeabilization solution and add 20 µl of FcR blocking solution and incubate for 5 min. (4°C, in the dark). - Perform intracellular staining of FoxP3 for 30 min. (4°C, in the dark) with appropriate amount of 3G3 antibody (final concentration 2-10 µg/ml; optimal concentration for peripheral blood is 3 µg/ml). - Add 1-2 ml of ice-cold permeabilization solution and incubate for 5 min. at 4°C in the dark. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 300 µl of ice-cold PBS with 1% BSA and measure on a FACS device with appropriate setting as soon as possible. Keep cold until measuring. (If necessary, for the same sample use mouse IgG1 isotype control (MOPC-21) in the same format as anti-FoxP3 antibody 3G3.) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Foxp3 |
| 全名 | forkhead box P3 |
| 背景介绍 | The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). [UniProt] Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. [UniProt] |
| 细胞定位 | Cytoplasm, Nucleus. [UniProt] |
| 产品亮点 | Related products: FOXP3 antibodies; FOXP3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs) |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
克隆号文献
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin.
IP / Rabbit
Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation.
IP / Mouse
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
WB / Rat
The basic helix-loop-helix/PAS factor Sim is associated with hsp90. Implications for regulation by interaction with partner factors.
IP / D. melanogaster
A tyrosine kinase-dependent pathway regulates ligand-dependent activation of the dioxin receptor in human keratinocytes.
IP / Human
All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a foldosome.
WB / Mouse
The 23-kDa acidic protein in reticulocyte lysate is the weakly bound component of the hsp foldosome that is required for assembly of the glucocorticoid receptor into a functional heterocomplex with hsp90.
WB / Rabbit
