ARG63507

anti-FOXP3 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μg

499

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Goat Polyclonal antibody recognizes FOXP3
反应物种	Ms
预测物种	Rat
应用	FACS, IHC-P
宿主	Goat
克隆	Polyclonal
同位型	IgG
靶点名称	FOXP3
抗原物种	Mouse
抗原	KRSQRPNKCSNP
偶联标记	Un-conjugated
別名	FOXP3; Forkhead Box P3; DIETER; XPID; AIID; PIDX; IPEX; JM2; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; SCURFIN; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; FOXP3delta7; Scurfin

应用说明

预测反应性说明

This antibody is predicted to react to rat FOXP3 based on the product citation paper. (https://doi.org/10.46235/1028-7221-1013-APO)

应用建议

应用	推荐稀释比
FACS	10 µg/ml
IHC-P	2 - 4 µg/ml

应用说明

IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from goat serum by antigen affinity chromatography.
缓冲液	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	0.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 20371 Mouse FOXP3 Swiss-port # Q99JB6 Mouse Forkhead box protein P3
基因名称	FOXP3
全名	forkhead box P3
背景介绍	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
生物功能	Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). [UniProt] Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. [UniProt]
细胞定位	Cytoplasm, Nucleus. [UniProt]
产品亮点	Related products: FOXP3 antibodies; FOXP3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs)
研究领域	Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Regulatory T cells Study antibody
预测分子量	47 kDa
翻译后修饰	Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

ARG63507 anti-FOXP3 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG63507 anti-FOXP3 antibody at 2 - 4 µg/ml dilution.
ARG63507 anti-FOXP3 antibody IHC-P image

Immunohistochemistry: Paraffin embedded Mouse Thymus (Right panel shows staining without primary antibody as negative control). (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63507 anti-FOXP3 antibody at 2 µg/ml dilution followed by HRP-staining.
ARG63507 anti-FOXP3 antibody FACS image

Flow Cytometry: Paraformaldehyde-fixed NIH/3T3 cells permeabilized with 0.5% Triton. Cells were stained with ARG63507 anti-FOXP3 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).