ARG64795
anti-Ferritin Light Chain antibody
anti-Ferritin Light Chain antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
产品描述 | Goat Polyclonal antibody recognizes Ferritin Light Chain |
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反应物种 | Hu, Ms, Rat |
预测物种 | Cow, Dog |
应用 | ELISA, IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Ferritin Light Chain |
抗原物种 | Human |
抗原 | C-GEYLFERLTLKHD |
偶联标记 | Un-conjugated |
別名 | NBIA3; Ferritin L subunit; Ferritin light chain; LFTD |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by antigen affinity chromatography. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008] |
研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
预测分子量 | 20 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG64795 anti-Ferritin Light Chain antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64795 anti-Ferritin Light Chain antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64795 anti-Ferritin Light Chain antibody IHC image
Immunohistochemistry: paraffin-embedded Human Brain Cortex (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64795 anti-Ferritin Light Chain antibody at 3.8 µg/ml dilution, followed by HRP-staining.
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ARG64795 anti-Ferritin Light Chain antibody WB image
Western blot: 35 µg of Human cerebellum (A), Human liver (B), Human placenta (C), Mouse brain (D) and Rat brain (E) lysates (in RIPA buffer) stained with ARG64795 anti-Ferritin Light Chain antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64795 anti-Ferritin Light Chain antibody ELISA image
Sandwich ELISA: Capture rabbit antibody at 5 µg/ml dilution combined with ARG64795 anti-Ferritin Light Chain antibody as a detection antibody at 1.5 µg/ml dilution. Results of a typical standard run with optical density.