ARG10749
anti-Fibrillarin antibody
anti-Fibrillarin antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Monkey
Gene Regulation antibody; Nucleolar Marker antibody; DFC Marker antibody; Dense fibrillar component Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Fibrillarin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Fibrillarin |
| 抗原物种 | Human |
| 抗原 | Full length Human Fibrillarin expressed in and purified from E. coli. |
| 偶联标记 | Un-conjugated |
| 別名 | rRNA 2'-O-methyltransferase fibrillarin; RNU3IP1; 34 kDa nucleolar scleroderma antigen; FIB; FLRN; EC 2.1.1.-; Histone-glutamine methyltransferase |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FBL |
| 全名 | fibrillarin |
| 背景介绍 | This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008] |
| 生物功能 | S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. [UniProt] |
| 研究领域 | Gene Regulation antibody; Nucleolar Marker antibody; DFC Marker antibody; Dense fibrillar component Marker antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). |
检测图片 (5) Click the Picture to Zoom In
-
ARG10749 anti-Fibrillarin antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG10749 anti-Fibrillarin antibody which binds to nucleoli (red). Cells are also co-stained in green with a chicken polyclonal antibody to vimentin. DNA is revealed with DAPI.
-
ARG10749 anti-Fibrillarin antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10749 anti-Fibrillarin antibody (red) at 1:5000 dilution and costained with ARG52468 anti-Vimentin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The Fibrillarin antibody detects protein expressed in nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.
-
ARG10749 anti-Fibrillarin antibody WB image
Western blot: HeLa cell lysate stained with ARG10749 anti-Fibrillarin antibody.
-
ARG10749 anti-Fibrillarin antibody WB image
Western blot: NIH/3T3, HEK293, HeLa, SH-SY5Y, C6 and COS1 cell lysates stained with ARG10749 anti-Fibrillarin antibody (green) at 1:5000 dilution.
-
ARG10749 anti-Fibrillarin antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10749 anti-Fibrillarin antibody (red) at 1:5000 dilution and costained with ARG52468 anti-Vimentin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The Fibrillarin antibody detects protein expressed in the nucleoli of cells, while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.
