ARG66162

Name: anti-Fibronectin antibody
Brand: arigo Biolaboratories
SKU: ARG66162
Price: 399 USD
Availability: InStock
Rating: 4.0 (1 reviews)

anti-Fibronectin antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μg

399
258

50 μg

279
181

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody recognizes Fibronectin
反应物种	Hu, Ms, Rat
应用	IHC-P, WB
宿主	Mouse
克隆	Monoclonal
同位型	IgG
靶点名称	Fibronectin
抗原物种	Human
抗原	Synthetic peptide from Human Fibronectin
偶联标记	Un-conjugated
別名	ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

应用说明

应用建议

应用	推荐稀释比
IHC-P	1:50 - 1:300
WB	1:1000 - 1:2000

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 14268 Mouse FN1 GeneID: 2335 Human FN1 Swiss-port # P02751 Human Fibronectin Swiss-port # P11276 Mouse Fibronectin
基因名称	FN1
全名	fibronectin 1
背景介绍	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
生物功能	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
产品亮点	Related Antibody Duos and Panels: ARG30346 Myofibroblast / Fibrosis Antibody Panel Related products: Fibronectin antibodies; Fibronectin ELISA Kits; Fibronectin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels for Myofibroblasts and CAFs
预测分子量	272 kDa
翻译后修饰	Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

检测图片 (9) Click the Picture to Zoom In

ARG66162 anti-Fibronectin antibody WB image

Western blot: 20 µg of Huh7 and Huh7 shHMGB1 cell lysates stained with ARG66162 anti-Fibronectin antibody (1:1000), ARG55914 anti-E-cadherin antibody (1:1000) and ARG65636 anti-HMGB1 antibody (1:2000).
ARG66162 anti-Fibronectin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.
ARG66162 anti-Fibronectin antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.
ARG66162 anti-Fibronectin antibody IHC image

Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.