ARG54079
anti-Fyn antibody
anti-Fyn antibody for ICC/IF,Western blot and Human,Mouse,Rat,Monkey
Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes FYN |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | Fyn |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Fyn protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | p59-FYN; Tyrosine-protein kinase Fyn; Src-like kinase; Proto-oncogene c-Fyn; p59-Fyn; Proto-oncogene Syn; SYN; SLK; EC 2.7.10.2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 59 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 10 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FYN |
| 全名 | FYN proto-oncogene, Src family tyrosine kinase |
| 背景介绍 | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival,cell adhesion,integrin-mediated signaling,cytoskeletal remodeling,cell motility,immune response and axon guidance.Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain.Following activation by PKA,the protein subsequently associates with PTK2/FAK1,allowing PTK2/FAK1 phosphorylation,activation and targeting to focal adhesions.Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT.Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage.Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1,KIRREL and TRPC6.Plays a role in neural processes by phosphorylating DPYSL2,a multifunctional adapter protein within the central nervous system,ARHGAP32,a regulator for Rho family GTPases implicated in various neural functions, and SNCA,a small pre-synaptic protein.Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation.Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1,thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts.CSK maintains LCK and FYN in an inactive form.Promotes CD28-induced phosphorylation of VAV1. |
| 生物功能 | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cell membrane. Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking. |
| 产品亮点 | Related Antibody Duos and Panels: ARG30169 Src Family Protein Tyrosine Kinases Antibody Panel Related products: Fyn antibodies; Fyn Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (By similarity). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Palmitoylation at Cys-3 and Cys-6 regulates subcellular location. |
检测图片 (2) Click the Picture to Zoom In
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ARG54079 anti-Fyn antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with -20°C Methanol and stained with ARG54079 anti-Fyn antibody at 1:50 dilution.
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ARG54079 anti-Fyn antibody WB image
Western blot: Jurkat cell lysate stained with ARG54079 anti-Fyn antibody at 1:500 dilution.
