ARG40829
anti-GAD2 / GAD65 antibody
anti-GAD2 / GAD65 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GAD2 / GAD65 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GAD2 / GAD65 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to K84-L182 of Human GAD65. |
| 偶联标记 | Un-conjugated |
| 別名 | Glutamate decarboxylase 65 kDa isoform; EC 4.1.1.15; GAD65; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GAD2 |
| 全名 | glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) |
| 背景介绍 | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| 生物功能 | Catalyzes the production of GABA. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. [UniProt] |
| 预测分子量 | 65 kDa |
| 翻译后修饰 | Phosphorylated; which does not affect kinetic parameters or subcellular location. Palmitoylated; which is required for presynaptic clustering. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40829 anti-GAD2 / GAD65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human glioma tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40829 anti-GAD2 / GAD65 antibody at 1 µg/ml, overnight at 4°C.
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ARG40829 anti-GAD2 / GAD65 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain and Mouse brain tissue lysates stained with ARG40829 anti-GAD2 / GAD65 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG40829 anti-GAD2 / GAD65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40829 anti-GAD2 / GAD65 antibody at 1 µg/ml, overnight at 4°C.
