ARG63756
anti-GAD2 / GAD65 antibody
anti-GAD2 / GAD65 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GAD2 / GAD65 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported variants. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GAD2 / GAD65 |
| 抗原物种 | Human |
| 抗原 | C-TLEDNEERMSRLSK |
| 偶联标记 | Un-conjugated |
| 別名 | Glutamate decarboxylase 65 kDa isoform; EC 4.1.1.15; GAD65; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| 研究领域 | Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 65 kDa |
| 翻译后修饰 | Phosphorylated; which does not affect kinetic parameters or subcellular location. Palmitoylated; which is required for presynaptic clustering. |
检测图片 (3) Click the Picture to Zoom In
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ARG63756 anti-GAD2 / GAD65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63756 anti-GAD2 / GAD65 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63756 anti-GAD2 / GAD65 antibody WB image
Western blot: 35 µg of Human brain (hippocampus) lysate (in RIPA buffer) stained with ARG63756 anti-GAD2 / GAD65 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63756 anti-GAD2 / GAD65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63756 anti-GAD2 / GAD65 antibody at 2.5 µg/ml dilution followed by AP-staining.
