ARG11126

anti-GFAP antibody [5C10]

anti-GFAP antibody [5C10] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Horse,Pig

概述

产品描述 Mouse Monoclonal antibody [5C10] recognizes GFAP
反应物种 Hu, Ms, Rat, Hrs, Pig
预测物种 Cow
应用 ICC/IF, IHC-Fr, WB
宿主 Mouse
克隆 Monoclonal
克隆号 5C10
同位型 IgG1
靶点名称 GFAP
抗原物种 Pig
抗原 Purified pig spinal cord GFAP.
偶联标记 Un-conjugated
別名 Glial fibrillary acidic protein; ALXDRD; GFAP

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:1000
IHC-Fr1:1000
WB1:5000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 50 kDa

属性

形式 Liquid
纯化 Purified
缓冲液 PBS, 5 mM Sodium azide and 50% Glycerol.
抗菌剂 5 mM Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

基因名称 GFAP
全名 glial fibrillary acidic protein
背景介绍 This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
生物功能 GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
细胞定位 Cytoplasm. Note=Associated with intermediate filaments. [UniProt]
预测分子量 50 kDa
翻译后修饰 Phosphorylated by PKN1. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG11126 anti-GFAP antibody [5C10] ICC/IF image

    Immunofluorescence: E20 rat cortical neuron-glial cells stained with ARG11126 anti-GFAP antibody [5C10] (red) at 1:1000 dilution, and co-stained with anti-Neurofilament NF-L antibody (red) at 1:2000 dilution. DAPI (blue) for nuclear staining.

  • ARG11126 anti-GFAP antibody [5C10] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG11126 anti-GFAP antibody [5C10] (green) at 1:1000 dilution, and co-stained with ARG10732 anti-Neurofilament NF-L antibody antibody (red) at 1:2000 dilution. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with antibodies.).

  • ARG11126 anti-GFAP antibody [5C10] WB image

    Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG11126 anti-GFAP antibody [5C10] at 1:2000 dilution.