ARG52313
anti-GFAP antibody
anti-GFAP antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Cat,Chicken,Human,Mouse,Rabbit,Rat,Rhesus Monkey
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
概述
产品描述 | Chicken Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP) |
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反应物种 | Hu, Ms, Rat, Bov, Cat, Chk, R. Mk, Rb |
应用 | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
宿主 | Chicken |
克隆 | Polyclonal |
同位型 | IgY |
靶点名称 | GFAP |
抗原物种 | Bovine |
抗原 | Recombinant and purified bovine GFAP |
偶联标记 | Un-conjugated |
別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
应用说明
应用建议 |
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应用说明 | Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Total IgY fraction |
缓冲液 | Total IgY fraction in PBS and 10 mM Sodium azide |
抗菌剂 | 10 mM Sodium azide |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | GFAP |
全名 | glial fibrillary acidic protein |
背景介绍 | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
生物功能 | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
产品亮点 | Related Antibody Duos and Panels: ARG30324 Neuroinflammation Antibody Panel Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Chicken IgY secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients Astrocyte-to-neuron conversion for Parkinson's disease treatment |
研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
预测分子量 | 50 kDa |
翻译后修饰 | Phosphorylated by PKN1. |
检测图片 (2) Click the Picture to Zoom In
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ARG52313 anti-GFAP antibody WB image
Western Blot: Rat cortex lysate showing specific immunolabeling of the ~50k GFAP protein stained with ARG52313 anti-GFAP antibody.
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ARG52313 anti-GFAP antibody ICC/IF image
Immunofluorescence: Mixed cultures of neurons and glia stained with ARG52313 anti-GFAP antibody (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.