ARG52313

anti-GFAP antibody

anti-GFAP antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Cat,Chicken,Human,Mouse,Rabbit,Rat,Rhesus Monkey

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody

概述

产品描述 Chicken Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)
反应物种 Hu, Ms, Rat, Bov, Cat, Chk, R. Mk, Rb
应用 ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
宿主 Chicken
克隆 Polyclonal
同位型 IgY
靶点名称 GFAP
抗原物种 Bovine
抗原 Recombinant and purified bovine GFAP
偶联标记 Un-conjugated
別名 Glial fibrillary acidic protein; ALXDRD; GFAP

应用说明

应用建议
应用 推荐稀释比
ELISA1:5000
FACS1:100
ICC/IF1:1000
IHC-Fr1:1000
IHC-P1:1000
WB1:10000
应用说明 Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Total IgY fraction
缓冲液 Total IgY fraction in PBS and 10 mM Sodium azide
抗菌剂 10 mM Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

基因名称 GFAP
全名 glial fibrillary acidic protein
背景介绍 GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
生物功能 GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30324 Neuroinflammation Antibody Panel
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Chicken IgY secondary antibodies;
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研究领域 Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
预测分子量 50 kDa
翻译后修饰 Phosphorylated by PKN1.

检测图片 (2) Click the Picture to Zoom In

  • ARG52313 anti-GFAP antibody WB image

    Western Blot: Rat cortex lysate showing specific immunolabeling of the ~50k GFAP protein stained with ARG52313 anti-GFAP antibody.

  • ARG52313 anti-GFAP antibody ICC/IF image

    Immunofluorescence: Mixed cultures of neurons and glia stained with ARG52313 anti-GFAP antibody (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.