ARG64066

anti-GFAP antibody

anti-GFAP antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
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publication_link 参考文献1

概述

产品描述 Goat Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)
反应物种 Hu, Ms, Rat
预测物种 Dog
应用 IHC-Fr, IHC-P, WB
特异性 GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 GFAP
抗原物种 Human
抗原 C-DGEVIKESKQEHKD
偶联标记 Un-conjugated
別名 Glial fibrillary acidic protein; ALXDRD; GFAP

应用说明

应用建议
应用 推荐稀释比
IHC-Fr0.01 µg/ml
IHC-PAssay - dependent
WB0.003 - 0.01 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

全名 glial fibrillary acidic protein
背景介绍 GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
生物功能 GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30007 Astrocyte Marker Antibody Duo (Host: Goat, Rabbit)
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
研究领域 Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
预测分子量 50 kDa
翻译后修饰 Phosphorylated by PKN1.

检测图片 (4) Click the Picture to Zoom In

  • ARG64066 anti-GFAP antibody WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG64066 anti-GFAP antibody at 1:5000 dilution.

  • ARG64066 anti-GFAP antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Human hypothalamus tissue stained with ARG64066 anti-GFAP antibody at 0.01 µg/ml dilution.

  • ARG64066 anti-GFAP antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64066 anti-GFAP antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64066 anti-GFAP antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64066 anti-GFAP antibody at 2 µg/ml dilution, followed by HRP-staining.

客户反馈

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anti-GFAP antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-GFAP antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:800

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Acupuncture promotes neurological recovery and regulates lymphatic function after acute inflammatory nerve root injury

IHC-Fr / Human

Jie Wang et al.
Heliyon.,  (2024)

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