ARG62991
anti-GFAP antibody [GF-01]
anti-GFAP antibody [GF-01] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Cat,Pig
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [GF-01] recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| 反应物种 | Hu, Cat, Pig |
| 不反应物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | The clone GF-01 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GF-01 |
| 同位型 | IgG1 |
| 靶点名称 | GFAP |
| 抗原物种 | Pig |
| 抗原 | Pellet of porcine brain cold-stable proteins after depolymerization of microtubules. |
| 偶联标记 | Un-conjugated |
| 別名 | Glial fibrillary acidic protein; ALXDRD; GFAP |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: The clone GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-P: Human brain (cortex, cerebellum) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 生物功能 | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| 产品亮点 | Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Phosphorylated by PKN1. |
检测图片 (2) Click the Picture to Zoom In
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ARG62991 anti-GFAP antibody [GF-01] IHC-P image
Immunohistochemistry: Human cerebellum (paraffin-embedded sections) stained with ARG62991 anti-GFAP antibody [GF-01].
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ARG62991 anti-GFAP antibody [GF-01] IHC-P image
Immunohistochemistry: Human brain cortex (paraffin-embedded sections) stained with ARG62991 anti-GFAP antibody [GF-01].
