ARG62343
anti-GFP antibody [GF28R]
anti-GFP antibody [GF28R] for Dot blot,ELISA,ICC/IF,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [GF28R] recognizes GFP |
|---|---|
| 反应物种 | Other |
| 应用 | Dot, ELISA, ICC/IF, IP, WB |
| 特异性 | Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GF28R |
| 同位型 | IgG2b |
| 靶点名称 | GFP |
| 抗原物种 | Others |
| 抗原 | GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A Purified |
| 纯化说明 | Protein A affinity chromatography from mouse ascites fluid. |
| 缓冲液 | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | In cell and molecular biology, the GFP protein (238aa, 26.9kDa) is frequently used as a reporter to analysis the protein expressing. In modified forms of GFP has been used to be biosensors, and many animals have been created GFP expressing model to analysis gene expression and biological function by transfecting GFP expressing gene into organisms or cells by breeding, injection with a viral vector, or cell transformation. In addition, the GFP gene has been transfected and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. [Modified from wikipedia] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30001 Tag Internal Control Antibody Duo (GFP, GAPDH) ARG30209 Fluorescent-Tags Antibody Panel Related products: GFP antibodies; GFP ELISA Kits; GFP Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG62343 anti-GFP antibody [GF28R] WB image
Western Blot: 1) HEK293 untransfected control, 2) 1 μg, 3) 10 μg of lysate from GFP-tagged expressing plasmid transfected HEK293 stained with ARG62343 anti-GFP antibody [GF28R] at 1:1000 (1 μg/mL) dilution.
文献引用
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