ARG41400
anti-GPX4 antibody
anti-GPX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Ferroptosis/Oxytosis Study antibody
参考文献8
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GPX4 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GPX4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human GPX4. |
| 偶联标记 | Un-conjugated |
| 別名 | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 20 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GPX4 |
| 全名 | glutathione peroxidase 4 |
| 背景介绍 | This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity). [UniProt] |
| 细胞定位 | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30337 Ferroptosis / Oxytosis Antibody Panel Related products: GPX4 antibodies; GPX4 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Ferroptosis/Oxytosis Antibody Panel is launched Therapeutic strategies against PDAC |
| 研究领域 | Ferroptosis/Oxytosis Study antibody |
| 预测分子量 | 22 kDa (mitochondrial) 20 kDa (cytoplasmic) |
检测图片 (3) Click the Picture to Zoom In
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ARG41400 anti-GPX4 antibody WB image
Western blot: 20 μg of Mouse testis and Rat testis lysates stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.
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ARG41400 anti-GPX4 antibody WB image
Western blot: Mouse Testis stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.
From Lipeng Li et al. Ecotoxicol Environ Saf (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 2F1.
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ARG41400 anti-GPX4 antibody WB image
Western blot: GBC-SD cells stained with ARG41400 anti-GPX4 antibody.
From Desen Fan et al. Heliyon (2024), doi: 10.1016/j.heliyon.2024.e30260, Fig. 2. E.
客户反馈
Good
anti-GPX4 antibody
Application：WB
Sample：
Sample Loading Amount： 20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：Over night
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-GPX4 antibody
Application：WB
Sample：MCF7
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Disrupting pro-survival and inflammatory pathways with dimethyl fumarate sensitizes chronic lymphocytic leukemia to cell death
WB / Human
Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells
WB / Human
Erythropoietin ameliorates cognitive dysfunction in mice with type 2 diabetes mellitus via inhibiting iron overload and ferroptosis
WB / Mouse
FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice
WB / Mouse
A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage
WB / Human
Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis
WB / Human
Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis
WB / Mouse
Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals
WB / Mouse
