ARG42603
anti-GSDME / DFNA5 antibody
anti-GSDME / DFNA5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GSDME / DFNA5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GSDME / DFNA5 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-260 of Human GSDME / DFNA5 (NP_004394.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Inversely correlated with estrogen receptor expression 1; Non-syndromic hearing impairment protein 5; ICERE-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse brain | ||||||||
| 实际分子量 | ~ 62 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O60443 Human Non-syndromic hearing impairment protein 5 Swiss-port # Q9Z2D3 Mouse Non-syndromic hearing impairment protein 5 homolog |
|---|---|
| 基因名称 | DFNA5 |
| 全名 | deafness, autosomal dominant 5 |
| 背景介绍 | Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688). [Gasdermin-E, N-terminal]: Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed:28459430, PubMed:27281216). Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed:28459430). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed:28045099). Exhibits bactericidal activity (PubMed:27281216). [UniProt] |
| 细胞定位 | Gasdermin-E, N-terminal: Cell membrane. Gasdermin-E: Cytoplasm, cytosol. [UniProt] |
| 预测分子量 | 55 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG42603 anti-GSDME / DFNA5 antibody ICC/IF image
Immunofluorescence: PC-12 cells stained with ARG42603 anti-GSDME / DFNA5 antibody at 1:50 dilution.
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ARG42603 anti-GSDME / DFNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG42603 anti-GSDME / DFNA5 antibody at 1:100 dilution.
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ARG42603 anti-GSDME / DFNA5 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG42603 anti-GSDME / DFNA5 antibody at 1:1000 dilution.
文献引用
GSDME in Endothelial Cells: Inducing Vascular Inflammation and Atherosclerosis via Mitochondrial Damage and STING Pathway Activation
IHC-Fr / Mouse
