ARG58348
anti-GW182 antibody
anti-GW182 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GW182 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GW182 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence within aa. 950-1050 of Human GW182 (NP_055309.2). |
| 偶联标记 | Un-conjugated |
| 別名 | GW182; Trinucleotide repeat-containing gene 6A protein; EMSY interactor protein; GW182 autoantigen; Glycine-tryptophan protein of 182 kDa; GW1; CAG repeat protein 26; CAGH26; Protein GW1; TNRC6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Rat brain | ||||||
| 实际分子量 | 180 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q3UHK8 Mouse Trinucleotide repeat-containing gene 6A protein Swiss-port # Q8NDV7 Human Trinucleotide repeat-containing gene 6A protein |
|---|---|
| 基因名称 | TNRC6A |
| 全名 | trinucleotide repeat containing 6A |
| 背景介绍 | This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes. [UniProt] |
| 细胞定位 | Cytoplasm, P-body. [UniProt] |
| 预测分子量 | 210 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG58348 anti-GW182 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58348 anti-GW182 antibody at 1:100 dilution.
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ARG58348 anti-GW182 antibody WB image
Western blot: 25 µg of Rat brain lysate stained with ARG58348 anti-GW182 antibody at 1:1000 dilution.
