ARG56688
anti-Galectin 3 antibody
anti-Galectin 3 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Galectin 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Galectin 3 |
| 抗原物种 | Human |
| 抗原 | E.coli derived Recombinant Human Galectin-3. (ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI) |
| 偶联标记 | Un-conjugated |
| 別名 | Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LGALS3 |
| 全名 | lectin, galactoside-binding, soluble, 3 |
| 背景介绍 | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014] |
| 生物功能 | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt] |
| 预测分子量 | 26 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG56688 anti-Galectin 3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas infiltrating ductal adenocarcinoma tissue. The recommended ARG56688 anti-Galectin 3 antibody concentration is 31.25 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56688 anti-Galectin 3 antibody WB image
Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56688 anti-Galectin 3 antibody, under reducing conditions.
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ARG56688 anti-Galectin 3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas infiltrating ductal adenocarcinoma tissue. The recommended ARG56688 anti-Galectin 3 antibody concentration is 31.25 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56688 anti-Galectin 3 antibody WB image
Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56688 anti-Galectin 3 antibody, under non-reducing conditions.
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ARG56688 anti-Galectin 3 antibody standard curve image
Sandwich ELISA: ARG56688 anti-Galectin 3 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56797 anti-Galectin 3 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
