ARG56797

anti-Galectin 3 antibody (Biotin)

anti-Galectin 3 antibody (Biotin) for ELISA,Western blot and Human

概述

产品描述 Biotin-conjugated Rabbit Polyclonal antibody recognizes Galectin 3
反应物种 Hu
应用 ELISA, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Galectin 3
抗原物种 Human
抗原 E.coli derived Recombinant Human Galectin-3.
(ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI)
偶联标记 Biotin
別名 Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56688 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3958 Human LGALS3

Swiss-port # P17931 Human Galectin-3

基因名称 LGALS3
全名 lectin, galactoside-binding, soluble, 3
背景介绍 This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
生物功能 Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt]
预测分子量 26 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG56797 anti-Galectin 3 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56797 anti-Galectin 3 antibody (Biotin), under reducing conditions.

  • ARG56797 anti-Galectin 3 antibody (Biotin) standard curve image

    Direct ELISA: ARG56797 anti-Galectin 3 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56797 anti-Galectin 3 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56797 anti-Galectin 3 antibody (Biotin), under non-reducing conditions.

  • ARG56797 anti-Galectin 3 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56797 anti-Galectin 3 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56688 anti-Galectin 3 antibody as a capture antibody. Results of a typical standard run with optical density.