ARG63828
anti-Ghrelin preproprotein antibody
anti-Ghrelin preproprotein antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Ghrelin preproprotein |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P |
| 特异性 | This antibody is expected to recognise all reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Ghrelin preproprotein |
| 抗原物种 | Human |
| 抗原 | C-QDILWEEAKEAPADK |
| 偶联标记 | Un-conjugated |
| 別名 | Motilin-related peptide; Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; MTLRP; Ghrelin; Protein M46 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008] |
| 产品亮点 | Related products: Anti-Goat IgG secondary antibodies; Related news: Wnt / beta-catenin signaling for gastric fundus specification |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 13 kDa |
| 翻译后修饰 | O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid (PubMed:10604470). Amidation of Leu-98 is essential for obestatin activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG63828 anti-Ghrelin preproprotein antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63828 anti-Ghrelin preproprotein antibody at 2 µg/ml dilution followed by AP-staining.
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ARG63828 anti-Ghrelin preproprotein antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63828 anti-Ghrelin preproprotein antibody at 2 µg/ml dilution followed by AP-staining.
