ARG22617
anti-Granzyme B antibody [GB11]
anti-Granzyme B antibody [GB11] for CyTOF®-candidate,ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human,Chimpanzee,Monkey,Rhesus Monkey
概述
| 产品描述 | Mouse Monoclonal antibody [GB11] recognizes Granzyme B This antibody recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B. |
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| 反应物种 | Hu, Chimp, Mk, R. Mk |
| 应用 | CyTOF®-candidate, ELISA, FACS, ICC/IF, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GB11 |
| 同位型 | IgG1 |
| 靶点名称 | Granzyme B |
| 抗原物种 | Human |
| 抗原 | Purified Human Granzyme B. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. Membrane permeabilisation is required for this application. ELISA: This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with a biotin conjugated antibody as detection reagent. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
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| 纯化 | Purification with Protein A. |
| 缓冲液 | Tris buffered saline and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | GZMB |
| 全名 | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| 背景介绍 | Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] |
| 生物功能 | Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt] |
| 产品亮点 | Related products: Granzyme B antibodies; Granzyme B ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies Examining CTL/NK-mediated cytotoxicity by ELISA Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| 预测分子量 | 28 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG22617 anti-Granzyme B antibody [GB11] standard curve image
Sandwich ELISA: ARG22617 anti-Granzyme B antibody [GB11] as a capture antibody and biotinylated Mouse anti Human granzyme B as a detection antibody with recombinant Human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. It results of a typical standard run with optical density reading at 450 nm. Human plasma samples are diluted at i:2 (green) and 1:4 (blue).
