ARG45567
anti-H-Ras antibody
anti-H-Ras antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes H-Ras |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | H-Ras |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to C-terminal region of human H-Ras. |
| 偶联标记 | Un-conjugated |
| 別名 | HRAS; Harvey rat sarcoma viral oncogene homolog; GTPase HRas; C-H-RAS; RASH1; C-HA-RAS1; C-BAS/HAS; HAMSV; Transforming protein p21; H-RASIDX; Ha-Ras; p21ras; CTLO; c-H-ras; H-Ras-1; HRAS1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 21 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HRAS |
| 全名 | Harvey rat sarcoma viral oncogene homolog |
| 背景介绍 | H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation. |
| 生物功能 | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. [UniProt] |
| 细胞定位 | Cell membrane; Cytoplasm; Golgi apparatus; Membrane; Nucleus. [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45567 anti-H-Ras antibody WB image
Western blot: HEPA stained with ARG45567 anti-H-Ras antibody.
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ARG45567 anti-H-Ras antibody WB image
Western blot: Rat brain stained with ARG45567 anti-H-Ras antibody.
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ARG45567 anti-H-Ras antibody IHC-P image
Immunohistochemistry: Mouse intestine stained with ARG45567 anti-H-Ras antibody at 1 μg/ml dilution.
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ARG45567 anti-H-Ras antibody WB image
Western blot: Mouse brain stained with ARG45567 anti-H-Ras antibody.
