ARG62338
anti-HA tag antibody [HA.C5]
anti-HA tag antibody [HA.C5] for Dot blot,ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Gene Regulation antibody
参考文献3
概述
| 产品描述 | Mouse Monoclonal antibody [HA.C5] recognizes HA tag |
|---|---|
| 反应物种 | Other |
| 应用 | Dot, ELISA, FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | HA.C5 |
| 同位型 | IgG3 |
| 靶点名称 | HA tag |
| 抗原物种 | Others |
| 抗原 | YPYDVPDYA (HA) synthetic peptide conjugated to KLH. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A Purified |
| 纯化说明 | Protein A affinity chromatography from mouse ascites fluid. |
| 缓冲液 | 10mM PBS (pH 7.2) and 0.05% Sodium azide. |
| 抗菌剂 | 0.05% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. Duo to HA-tag is from influenza virus and not be found in nomal anamils, it is useful for the protein functional analysis when the specific antibodies for the protein-of-interest are unavailable. |
|---|---|
| 产品亮点 | Related products: HA tag antibodies; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG62338 anti-HA tag antibody [HA.C5] WB image
Western blot: 10 µg of 293T and 293T + myc-HA-mCherry cell lysates stained with ARG62338 anti-HA tag antibody [HA.C5] at 1:1000 dilutioin.
客户反馈
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anti-HA tag antibody [HA.C5]
Application：WB
Sample：293T and 293T + myc-HA-mCherry
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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