ARG65666

anti-HBV preS2 antibody [SQab1507]

Datasheet

参考文献2

订购信息

包装

价格 (USD)

100 μg

399
350

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [SQab1507] recognizes HBV preS2
反应物种	HBV
应用	ELISA, ICC/IF, IHC-P, WB
特异性	This antibody could recognize clinical samples including HBsAg (L-HBs and M-HBs) in serum, plasma, and tissue specimens. The antibody does not react with S-HBs protein.
宿主	Mouse
克隆	Monoclonal
克隆号	SQab1507
同位型	IgG1
靶点名称	HBV preS2
抗原物种	HBV
抗原	GST-tagged fusion protein around aa. 31-55 of HBV preS2
偶联标记	Un-conjugated
別名	large S protein; pre-S1/pre-S2/S; L glycoprotein; L-HBsAG; LHB; large surface protein; major surface antigen

应用说明

应用建议

应用	推荐稀释比
ELISA	1:5000-1:10000
ICC/IF	1:250-1:1000
IHC-P	1:200-1:350
WB	1:2500-1:6000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂	0.01% Thimerosal
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 944569 HBV S
基因名称	S
全名	L-HBsAG
背景介绍	Hepatitis B virus (HBV) is a hepadnavirus which has a circular genome composed of partially double-stranded DNA. The HBV surface protein antigens (HBsAg) are comprised of large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs contains preS1, prS2, and small protein. MHBs does not include preS1 protein and SHBs dose not include preS1 and preS2 proteins. HbsAg and its antibodies have been developed as biomarkers to monitor infection stage. Expression of preS1 and preS2 in tissue or serum are also important to reveal the mechanism of HBV infection.
生物功能	The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. [UniProt]
研究领域	Cancer antibody; Microbiology and Infectious Disease antibody

检测图片 (2) Click the Picture to Zoom In

ARG65666 anti-HBV preS2 antibody [SQab1507] WB image

Western blot: 1) HBV surface protein expressed 293T cell lysate and 2) 293T cell lysate (Mock) stained with ARG65665 anti-HBV preS1 antibody [SQab1506] (left) or ARG65666 anti-HBV preS2 antibody [SQab1507] (right) at 1:2500 dilution.

Data showed ARG65665 anti-HBV preS1 antibody [SQab1506] reacts with L-HBs protein only and ARG65666 anti-HBV preS2 antibody [SQab1507] reacts with L-HBs and M-HBs proteins.
ARG65666 anti-HBV preS2 antibody [SQab1507] ELISA image

Direct ELISA: Direct ELISA data shows that ARG65666 anti-HBV preS2 antibody [SQab1507] reacts with HBV L-HBs protein but not preS1 fragment, S-HBs protein and BSA.