ARG59655
anti-HDGF antibody
anti-HDGF antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes HDGF |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | HDGF |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 61-97 of Human HDGF. (KDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKA) |
| 偶联标记 | Un-conjugated |
| 別名 | HMG1L2; HMG-1L2; HDGF; High mobility group protein 1-like 2; Hepatoma-derived growth factor |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HDGF |
| 全名 | hepatoma-derived growth factor |
| 背景介绍 | HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016] |
| 生物功能 | HDGF Isoform 1: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618). Isoform 2: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719). Isoform 3: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719). [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. [UniProt] |
| 产品亮点 | Related products: HDGF antibodies; HDGF ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| 预测分子量 | 27 kDa |
| 翻译后修饰 | Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
-
ARG59655 anti-HDGF antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG59655 anti-HDGF antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
-
ARG59655 anti-HDGF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver stained with ARG59655 anti-HDGF antibody at 1 µg/ml dilution.
-
ARG59655 anti-HDGF antibody WB image
Western blot: Rat liver and 22RV1 whole cell lysates stained with ARG59655 anti-HDGF antibody at 0.5 µg/ml dilution.
-
ARG59655 anti-HDGF antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59655 anti-HDGF antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG59655 anti-HDGF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver stained with ARG59655 anti-HDGF antibody at 1 µg/ml dilution.
-
ARG59655 anti-HDGF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG59655 anti-HDGF antibody at 1 µg/ml dilution.
-
ARG59655 anti-HDGF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG59655 anti-HDGF antibody at 1 µg/ml dilution.
